Quantitation of thin-layer chromatograms with an Apple II computer-based videodensitometer
نویسندگان
چکیده
منابع مشابه
Quantitation of thin-layer chromatograms with an Apple II computer-based videodensitometer.
An inexpensive, high-speed densitometer made from an Apple II computer, a black-and-white video camera, and an image digitizing board is described. By supplementing the computer with a very fast coprocessor, one can obtain a measurement of a typical thin-layer spot in 30-40 s. Only minimal electronic expertise is required for assembling the system from the commercially available components. Coe...
متن کاملUse of nile red for the rapid in situ quantitation of lipids on thin-layer chromatograms.
We describe the use of the fluorescent dye nile red, 9-diethylamino-5H-benzo[alpha]phenoxazine-5-one, as a general-purpose reagent for the rapid detection and quantitation of a wide variety of lipids and other hydrophobic compounds separated by thin-layer chromatography. After samples are applied to silica gel plates and chromatographed, the plate is briefly dipped into a nile red solution (8 m...
متن کاملSpray detection of phospholipids on thin-layer chromatograms.
A new spray reagent with copper, ammonium molybdate, and sulphuric acid for the detection of phospholipids on thin-layer chromatograms is described. The specificity and sensitivity of the spray reagent is also shown.
متن کاملPhosphorus determination in phosphoglycerides from thin-layer chromatograms ARTHUR
Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail ...
متن کاملTime resolved chromatograms in ultra-thin layer chromatography.
Ultrathin-layer chromatography (UTLC) is a recently developed analytical method intended for compact, rapid separations of nanolitre analyte volumes. Optimizing this method's performance requires new measurement techniques compatible with the millimetre length scales and rapid separation dynamics observed in UTLC. We have designed, implemented and characterized a measurement system which record...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1985
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90523-8